Quantitative In Vivo Monitoring of Hypoxia and Vascularization of Patient-Derived Murine Xenografts of Mantle Cell Lymphoma Using Photoacoustic and Ultrasound Imaging

Tumor oxygenation and vascularization are important parameters that determine the aggressiveness of tumor its resistance to cancer therapies. We introduce dual-modality ultrasound photoacoustic imaging (US-PAI) for direct, non-invasive real-time in vivo evaluation patient-derived xenografts (PDXs) B-cell mantle cell lymphomas. The different optical properties oxyhemoglobin deoxyhemoglobin make it possible oxygen saturation (sO2) tissues using PAI. High-frequency color Doppler enables visualization blood flow with high resolution. were studied during growth three subcutaneously implanted xenograft (PDX) lymphomas (VFN-M1, VFN-M2 VFN-M5 R1). Similar values sO2 (sO2 Vital), determined from US-PAI volumetric analysis, obtained small large VFN-M1 tumors ranging 37.9 ± 2.2 40.5 6.0 Vital (%) 37.5 4.0 35.7 4.6 PDXs. In contrast, higher 57.1 4.8 40.8 5.7 (small large) R1 corresponds PDX model. percentage (assessed as micro-vessel areas) VFN-M1, by (2.8 0.1%, 3.8 0.8% 10.3 2.7%) large-stage clearly their diverse aggressiveness. data validated histology. conclusion, rapidly accurately provided relevant reproducible information on tissue real time without need a contrast agent.